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This research proposes and evaluates two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus (MPXV) with excessive specificity and sensitivity.
An ongoing multicountry outbreak of monkeypox was reported in Could 2022 with a number of deaths, affecting 107 nations of all six World Well being Group (WHO) areas. New diagnostic assays are wanted for monkeypox because of the present worldwide outbreak.
The authors of this text developed two real-time PCR assays focusing on endemic clades and the outbreak pressure of MPXV. The primers and probes had good diagnostic accuracy at distinguishing strains.
This assay supplies a dependable and easy methodology to quickly determine MPXV and differentiate the pressure within the ongoing monkeypox outbreak.
Supply:
Journal reference:
Huo, S., et al. (2023) Improvement of two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus IIa, IIb, and I clades and the B.1 lineage. Biosafety and Well being. doi.org/10.1016/j.bsheal.2022.10.005.
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